[ PROPERTIES ]
Host: E.coli
Residues: Ile86~Val259
Tags: Two N-terminal Tags, His-tag and T7-tag
Subcellular Location: Cell membrane; Single-passtype II membrane protein.
Purity: >90%
Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 5.1
Predicted Molecular Mass: 25.1kDa
Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducingconditions
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note:The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.ITLLIFTG AFLLGYVAFR GSCQACGDSV LVVDEDVNPE DSGRTTLYWS DLQAMFLRFLGEGRMEDTIR LTSLRERVAG SARMATLVQD ILDKLSRQKL DHVWTDTHYV GLQFPDPAHANTLHWVDADG SVQEQLPLED PEVYCPYSAT GNATGKLVYA HYGRSEDLQD LKAKGV
[ REFERENCES ]
1. Fleming R.E., et al. (2000) Proc. Natl. Acad. Sci. U.S.A. 97:2214-2219.
2. Kawabata H., et al. (2001) Blood 98:1949-1954.
3. Carninci P., et al. (2005) Science 309:1559-1563.
4. The MGC Project Team. (2004) Genome Res. 14:2121-2127.
[ IDENTIFICATION ]
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