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Biotinylated Human VEGF121 Protein, Avitag™,His Tag

Biotinylated Human VEGF121 Protein, Avitag™,His Tag
别名: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
储存条件: -20℃ 
Biotinylated Human VEGF121 Protein, Avitag™,His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VE1-H82E7-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Biotinylated Human VEGF121 Protein, Avitag™,His Tag

分子别名(Synonym)

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统(Source)

MABSol® Biotinylated Human VEGF121, Avitag,His Tag  is expressed from human HEK293 cells. It contains AA Ala 27 - Arg 147 

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 16.7 kDa. As a result of glycosylation, the protein migrates as 18 kDa and 22 kDa under reducing (R) condition, and 35-40 kDa under non-reducing (NR) condition (SDS-PAGE).

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human VEGF121, Avitag,His Tag (Cat. No. VE1-H82E7) SDS-PAGE gel

Biotinylated Human VEGF121, Avitag,His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized VEGFR1/R2-Fc at 1 μg/mL (100 μL/well) can bind Biotinylated Human VEGF121, Avitag,His Tag with a linear range of 0.1-2 ng/mL (QC tested).

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