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Unconjugated Human PDGF-BB Protein, His,Avitag™

Unconjugated Human PDGF-BB Protein, His,Avitag™
别名: PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
储存条件: -20℃ 
Unconjugated Human PDGF-BB Protein, His,Avitag™
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-PDB-H5127-50ug 50ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

Unconjugated Human PDGF-BB Protein, His,Avitag™

分子别名(Synonym)

PDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis

表达区间及表达系统(Source)

Unconjugated Human PDGF-BB, His,Avitag  is expressed from E.coli cells. It contains AA Ser 82 - Thr 190.

Predicted N-terminus: Met

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 15.1 kDa. the protein migrates as 17 kDa under reducing (R) condition, and 35 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Unconjugated Human PDGF-BB, His,Avitag (Cat. No. PDB-H5127) SDS-PAGE gel

Unconjugated Human PDGF-BB, His,Avitag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Unconjugated Human PDGF-BB, His,Avitag at 1 μg/mL (100 μL/well) can bind Human PDGF R beta, Fc Tag with a linear range of 0.5-16 ng/mL (QC tested).

 

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