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| 别名: | RANKL,CD254,TRANCE,OPGL,ODF | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human TNFSF11 / RANKL / CD254 Protein, His Tag (active trimer) (MALS verified)
RANKL,CD254,TRANCE,OPGL,ODF
Human TNFSF11, His Tag (active trimer) is expressed from human 293 cells. It contains AA Gly 64 - Asp 245.
Predicted N-terminus: His
Request for sequence
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 22.4 kDa. The protein migrates as 27-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human TNFSF11, His Tag (active trimer) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human TNFSF11, His Tag (active trimer)s more than 85% and the molecular weight of this protein is around 80-100 kDa verified by SEC-MALS.
Report
Immobilized Human RANK, Mouse IgG2a Fc Tag, low endotoxinat 0.5 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) with a linear range of 0.4-6 ng/mL (QC tested).
Protocol
Immobilized Human OPG, Fc Tag at 2 μg/mL (100 μL/well) can bind Human TNFSF11, His Tag (active trimer) with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Anti-TNFSF11 (human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.274 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxinon CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.309 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-TNFSF11 MAb (human IgG1) on AHC Biosensor, can bind Human TNFSF11, His Tag (active trimer) with an affinity constant of 0.239 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
