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| 别名: | CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 密度: | |
| 储存条件: | -20℃ |
Human CD40 Ligand / TNFSF5 Protein, Fc Tag (active trimer) (MALS verified)
CD40LG,CD154,CD40L,HIGM1,IGM,IMD3,T-BAM,TNFSF5,TRAP,gp39
Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) is expressed from human 293 cells . It contains AA Gly 116 - Leu 261 rimer Design.
Predicted N-terminus: Pro
This protein carries a human IgG1 Fc tag at the N-terminus.
The protein has a calculated MW of 74.7 kDa. The protein migrates as 120-150 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD40 Ligand, Fc Tag (active trimer) (MALS verified)is more than 90% in HP-SEC, and the molecular weight of this protein is around 160-180 kDa verified by SEC-MALS.
Report
Immobilized Human CD40, His Tag at 1 μg/mL (100 μL/well) can bind Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) with a linear range of 0.2-3 ng/mL (QC tested).
Protocol
Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) stimulates secretion of IL-6 by human B cells. The ED50 for this effect is 9.68-11.48 ng/mL in the presence of 5 ng/mL of Recombinant Human IL‑4 (Routinely tested).
Protocol
Neutralization assay shows that the cytokine secretion effect of Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) was inhibited by increasing concentration of anti-CD40L mAb. The concentration of CD40L used is 50 ng/mL. The EC50 is 0.051-0.058 μg/mL (Routinely tested).
Protocol
FACS analysis shows that Human CD40 Ligand, Fc Tag (active trimer) (MALS verified) can bind to 293T-CD40 cells surface CD40. The concentration of Human CD40 Ligand is 0.3 μg/mL (Routinely tested).
