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| 别名: | C5a,Complement Component 5a | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
|---|
| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human Complement C5a Protein, Tag Free
C5a,Complement Component 5a
Human Complement C5a, Tag Frees expressed from E.coli cells. It contains AA Leu 679 - Arg 751 .
Predicted N-terminus: Met
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 8.3 kDa. The protein migrates as 10-12 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human Complement C5a, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human Complement C5a, Tag Free at 2 μg/mL (100 μL/well) can bind Anti-C5a MAb, Human IgG1 with a linear range of 0.8-13 ng/mL (QC tested).
Protocol
Human Complement C5a, Tag Free induce N-acetyl-β-D-glucosaminidase release from differentiated U937 cells. The ED50 for this effect is 0.215-0.323 μg/mL (Routinely tested).
