载脂蛋白A4(APOA4)重组蛋白 (Mouse，小鼠2) _重组蛋白_蛋白组学_生命科学

载脂蛋白A4(APOA4)重组蛋白 (Mouse，小鼠2)

Recombinant Apolipoprotein A4 (APOA4)

别名:	ApoA-IV; ApoAIV; Apo-A4
		储存条件:	在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

货号	规格	可用库存	销售价(RMB)	您的折扣价(RMB)	购买数量
ZY65892Mu2P-10μg	10μg	现货	~~672.00~~	672

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熔点:
密度:
储存条件:	在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

载脂蛋白A4(APOA4)重组蛋白

规格及价格：

规格	价格
10µg	672
50µg	1680
200µg	3360
1mg	10080

[ PROPERTIES ]

Host: E.coli

Residues: Leu135~Asn281 (Accession # P06728),with two N-terminal Tags, His-tag and S-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 1mM DTT, 5% trehalose, 0.01%sarcosyl and preservative

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.2

Predicted Molecular Mass: 22.5kDa

Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducingconditions.

Note: The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins fromthe samegene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedtogive the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag andS-tag, itssequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- LKPYAVDLQDQINTQT QEMKLQLTPY IQRMQTTIKE NVDNLHTSMM PLATNLKDKF NRNMEELKGHLTPRANELKA TIDQNLEDLR RSLAPLTVGV QEKLNHQMEG LAFQMKKNAE ELQTKVSAKIDQLQKNLAPL VEDVQSKVKG N

[ IDENTIFICATION ]

[ REFERENCES ]

1. Williams S.C., et al. (1986) Mol. Cell. Biol. 6:3807-3814.

2. Reue K., Leete T.H. (1991) J. Biol. Chem. 266:12715-12721.

3. Puppione D.L., et al. (2006) Biochim. Biophys. Acta 1764:1363-1371.

4. Sauvaget D., et al. (2002) J. Biol. Chem. 277:34540-34548.

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载脂蛋白A4(APOA4)重组蛋白 (Mouse，小鼠2)