内皮脂肪酶(LIPG)重组蛋白(Rat，大鼠) _重组蛋白_蛋白组学_生命科学

内皮脂肪酶(LIPG)重组蛋白(Rat，大鼠)

Recombinant Lipase, Endothelial (LIPG)

别名:	EL; EDL; Endothelial Lipase; Endothelial cell-derived lipase
		储存条件:	在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

货号	规格	可用库存	销售价(RMB)	您的折扣价(RMB)	购买数量
ZY61690RaP-10μg	10μg	现货	~~1061.00~~	1061

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熔点:
密度:
储存条件:	在 2 至 8 °C 下储存一周。在 -20 至 -80 °C 下储存自收到之日起十二个月。

内皮脂肪酶(LIPG)重组蛋白

规格及价格：

规格	价格
10µg	1061
50µg	2652
200µg	5304
1mg	15912

[ PROPERTIES ]

Host: E.coli

Residues: Gly203~Lys471 ,with two N-terminal Tags, His-tag and T7-tag.

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg(determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris,500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.2

Predicted Molecular Mass: 34.6kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate of thetargetprotein. The loss rate was determined by accelerated thermal degradationtest,that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GVDINRRL SPDDADFVDVLHTYTLSFGL SIGIRMPVGH IDIYPNGGDF QPGCGFNDVM GSFAYGTISE MVKCEHERAVHLFVDSLVNQ DKPSFAFQCT DPNRFKRGIC LSCRKNRCNN IGYNAKKMRK KRNSKMYLKTRAGMPFRVYH YQLKVHMFSY KNSGDIQPDL YITLYGSNAD SQNLPLEIVE KIELNATNTFLVYTEEYLGD LFKIRLTWEG VSSSWYNLWN EFRSYLSQPS SPSRELHIRR IRVKSGETQRKVAFCVQDPM K

[ REFERENCES ]

1. Shimokawa Y., et al. (2005) Cardiovasc. Res. 66:594-600.

2. Bonne A.C.M., et al. (2001) DNA Seq. 12:285-287.

3. Stevenson F.T., et al. (2001) Kidney Int. 59:2062-2068.

4. Hutter C.M., et al. (2006) Atherosclerosis 185:78-86.

[ IDENTIFICATION ]

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内皮脂肪酶(LIPG)重组蛋白(Rat，大鼠)