胱天蛋白酶7(CASP7)重组蛋白(Rat，大鼠)

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Met1~Asn250

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: F7F246

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.2

Predicted Molecular Mass: 31.6kDa

Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation: 

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile ddH₂O.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

MTDDQDCAAE LEMADSSTED GVDAKPDRST IISSLLWKKK KNASMCPVST TRDRVPTYLY

RMDFEKMGKC IIINNKNFDK ATGMDVRNGT DKDAEALFKC FRSLGFEVTV YNDCSCAKMQ

DLLRRASEED HSNSACFACV LLSHGEENLI YGKDGVTPIK DLTAHFRGDR CKTLLEKPKL

FFIQACRGTE LDDGIQADSG PINDTDANPR YKIPVEADFL FAYSTVPGXX SWRNPGKGSW

FVQALCSILN

[ IDENTIFICATION ]

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