[ PROPERTIES ]
Accession: P55284
Host: E.coli
Residues: Gly67~Gly390
Tags: Two N-terminal Tags, His-tag and T7-tag
Subcellular Location: Cell junction. Cell
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.3
Predicted Molecular Mass: 40.4kDa
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test:
The thermal stability is described by the loss rate of the target
protein. The loss rate was determined by accelerated thermal degradation test,
that is, incubate the protein at 37oC for 48h, and no obvious degradation and
precipitation were observed. (Referring from China Biological Products Standard,
which was calculated by the Arrhenius equation.) The loss of this protein is less
than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The sequence of the target protein is listed below.
GKIK SNVNRQNAKY VLQGEFAGKI FGVDANTGNV LAYERLDREK VSEYFLTALI
VDKNTNKNLE QPSSFTVKVH DINDNWPVFS HQVFNASVPE MSAIGTSVIR VTAVDADDPT
VAGHATVLYQ IVKGNEYFSI DNSGLIFTKI KNLDREKQAE YKIVVETQDA LGLRGESGTA
TVMIRLEDIN DNFPVFTQST YTFSVPEDIR VGKPLGFLTV VDPDEPQNRM TKYSIMQGEY
RDTFTIETDP KRNEGIIKPT KSLDYEVIQQ YTFYIEATDP TIRYEYLSST SGKNKAMVTI
NVLDVDEPPV FQRHFYHFKL PENQKKPLIG
[ IDENTIFICATION ]
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