[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Met1~Ser178
Tags: N-terminal His Tag
Tissue Specificity: Blood.
Subcellular Location: Cytoplasm. Cytoplasmic vesicle.
Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 21.9kDa
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
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