Anti-Cyclin D1 antibody
-
概述
- 产品描述Cyclin D1 belongs to the family of cyclin proteins which function as the regulatory subunits of cyclin/cyclin dependent kinase holoenzymes. The cyclin D1 proto-oncogene is an important regulator of G1 to S-phase transition and an important cofactor for several transcription factors in numerous cell types. Cyclin D1 is known to modulate local chromatin structure and transcription of genes involved in proliferation and differentiation through CDK-independent association with histone acetylases and deacetylases.
- 产品名称Anti-Cyclin D1 antibody
- 分子量34 kDa
- 种属反应性Human,Mouse,Rat,Zebrafish
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of C-terminal residues of cyclin D1.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm, Membrane, Nucleus.
- 数据链接SwissProt: P24385 Human
SwissProt: P25322 Mouse
SwissProt: P39948 Rat
- 其它名称AI327039 antibody
B cell CLL/lymphoma 1 antibody
B cell leukemia 1 antibody
B cell lymphoma 1 protein antibody
B-cell lymphoma 1 protein antibody
BCL 1 antibody
BCL-1 antibody
BCL-1 oncogene antibody
BCL1 antibody
BCL1 oncogene antibody
ccnd1 antibody
CCND1/FSTL3 fusion gene, included antibody
CCND1/IGHG1 fusion gene, included antibody
CCND1/IGLC1 fusion gene, included antibody
CCND1/PTH fusion gene, included antibody
CCND1_HUMAN antibody
cD1 antibody
Cyl 1 antibody
D11S287E antibody
G1/S specific cyclin D1 antibody
G1/S-specific cyclin-D1 antibody
Parathyroid adenomatosis 1 antibody
PRAD1 antibody
PRAD1 oncogene antibody
U21B31 antibody
more
-
应用
WB:1:5,000
ICC:1:200
FC:1:50-1:100
-
Fig1: Western blot analysis of Cyclin D1 on MCF-7 lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: ICC staining Cyclin D1 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig3: ICC staining Cyclin D1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig4: ICC staining Cyclin D1 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.
Fig5: Flow cytometric analysis of Cyclin D1 was done on HepG2 cells. The cells were fixed, permeabilized and stained with Cyclin D1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
