Anti-Osteopontin antibody
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概述
- 产品描述Osteopontin (OPN), also known as bone sialoprotein I (BSP-1 or BNSP), early T-lymphocyte activation (ETA-1), secreted phosphoprotein 1 (SPP1), 2ar and Rickettsia resistance (Ric),[5] is a protein that in humans is encoded by the SPP1 gene (secreted phosphoprotein 1). The murine ortholog is Spp1. Osteopontin is a SIBLING (glycoprotein) that was first identified in 1986 in osteoblasts. The prefix osteo- indicates that the protein is expressed in bone, although it is also expressed in other tissues. The suffix -pontin is derived from "pons," the Latin word for bridge, and signifies osteopontin's role as a linking protein. Osteopontin is an extracellular structural protein and therefore an organic component of bone. Synonyms for this protein include sialoprotein I and 44K BPP (bone phosphoprotein). The gene has 7 exons, spans 5 kilobases in length and in humans it is located on the long arm of chromosome 4 region 22 (4q1322.1). The protein is composed of ~300 amino acids residues and has ~30 carbohydrate residues attached, including 10 sialic acid residues, which are attached to the protein during post-translational modification in the Golgi apparatus. The protein is rich in acidic residues: 30-36% are either aspartic or glutamic acid.
- 产品名称Anti-Osteopontin antibody
- 分子量60 kDa
- 种属反应性Human, Zebrafish
- 验证应用WB, IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human aa 280-310.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytosol
- 数据链接SwissProt: P10451 Human
- 其它名称BNSP antibody
Bone sialoprotein 1 antibody
BSP I antibody
BSPI antibody
Early T lymphocyte activation 1 antibody
ETA 1 antibody
ETA1 antibody
MGC110940 antibody
Nephropontin antibody
OPN antibody
Osteopontin antibody
osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein antibody
OSTP_HUMAN antibody
PSEC0156 antibody
secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1) antibody
Secreted phosphoprotein 1 antibody
SPP 1 antibody
SPP-1 antibody
SPP1 antibody
SPP1/CALPHA1 fusion antibody
Urinary stone protein antibody
Uropontin antibody
more
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Fig1: Western blot analysis on human thymus using anti-Osteopontin polyclonal antibody.
Fig2: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Osteopontin antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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