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Anti-ICAM1/CD54 antibody
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
Fig1: Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-CD54 t 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig2: Sample:
Hela(Human) Cell Lysate at 30 ug
Hela KO ICAM1 (Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1/CD54 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig3: Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Fig4: Blank control: HUVEC cells(blue).
Primary Antibody:Rabbit Anti-CD54 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (ER1910-98, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.
Fig5: Blank control: mouse thymouses(blue)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA(green).
Fig6: Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-ICAM1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
Fig7: Blank control (blue line): A431 cells(blue).
Primary Antibody (green line): Rabbit Anti-ICAM1/PE-CY7 Conjugated antibody (ER1910-98-PE-CY7)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Pro
Fig8: Sample:
Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56 kD
Fig9: Sample:
Spleen (Mouse) Lysate at 40 ug
Primary: Anti-ICAM1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 56/69 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!