Anti-ICAM-2/CD102 antibody

Anti-ICAM-2/CD102 antibody

• 概述

• 产品描述ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance.
• 产品名称Anti-ICAM-2/CD102 antibody
• 分子量28 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ICAM-2 175-277/277
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Single-pass type I membrane protein.
• 其它名称CD102
  Intercellular adhesion molecule 2
  CD102 antigen
  ICAM 2
  ICAM-2
  ICAM2
  Intercellular adhesion molecule 2 precursor
  Ly60.
  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

•  

  

  Fig1: Blank control: Jurkat cells(blue).
  Primary Antibody:Rabbit Anti-CD102 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Molt-4(Human) Cell Lysate at 30 ug
  K562(Human) Cell Lysate at 30 ug
  Primary: Anti- ICAM-2/CD102) at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 28 kD
  Observed band size: 31 kD

   

  

  Fig3: Sample:
  Jurkat(Human) Cell Lysate at 30 ug
  Primary: Anti-CD102 (ER1910-99) at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 28 kD
  Observed band size: 28 kD

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