Anti-IL-12 antibody
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概述
- 产品描述Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated Killer cells, and stimulate the production of IFN-gamma by resting PBMC.
- 产品名称Anti-IL-12 antibody
- 分子量22 KDa
- 种属反应性Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from rat IL-12 141-200/215
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Secreted.
- 其它名称IL12
IL-12
CLMF p35
CLMF1
CTL maturation factor (TcMF)
Cytotoxic lymphocyte maturation factor 1
Cytotoxic lymphocyte maturation factor 35 kDa subunit
IL 12 subunit p35
IL12A
Interleukin 12 alpha chain
Interleukin 12 p35
Interleukin 12A
Natural killer cell stimulatory factor 1
CLMF
CLMF p35
Cytotoxic lymphocyte maturation factor 35 kDa subunit
IL-12 subunit p35
IL-12A
IL12 subunit p35
IL12A
IL12A_HUMAN
Interleukin 12 p35
Interleukin 12A
Interleukin-12 subunit alpha
NFSK
NK cell stimulatory factor chain 1
NKSF1. NK cell stimulatory factor chain 1
NKSF1
p35
IL12A_RAT.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Liver(Rat) Lysate at 40 ug
Primary: Anti-IL-12 at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 24 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (IL-12) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!