Anti-NF-κB p65 antibody

Anti-NF-κB p65 antibody

• 概述

• 产品描述NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins. NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression.
• 产品名称Anti-NF-κB p65 antibody
• 分子量65 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to N-terminal NF-κB p65.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Nucleus, cytoplasm
• 其它名称Avian reticuloendotheliosis viral (v rel) oncogene homolog A antibody
  MGC131774 antibody
  NF kappa B p65delta3 antibody
  NFKB3 antibody
  Nuclear Factor NF Kappa B p65 Subunit antibody
  Nuclear factor NF-kappa-B p65 subunit antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B cells 3 antibody
  Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 antibody
  OTTHUMP00000233473 antibody
  OTTHUMP00000233474 antibody
  OTTHUMP00000233475 antibody
  OTTHUMP00000233476 antibody
  OTTHUMP00000233900 antibody
  p65 antibody
  p65 NF kappaB antibody
  p65 NFkB antibody
  relA antibody
  TF65_HUMAN antibody
  Transcription factor p65 antibody
  v rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B cells 3 (p65)) antibody
  V rel avian reticuloendotheliosis viral oncogene homolog A antibody
  v rel reticuloendotheliosis viral oncogene homolog A (avian) antibody
  V rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B cells 3, p65 antibody
  more

• 应用

  WB: 1:1,000-1:2,000
  IHC-P: 1:100-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of NF-κB p65 on different lysates using anti-NF-κB p65 antibody at 1/1000 dilution.
  Positive control:
  Lane 1: Hela
  Lane 2: A549
  Lane 3: PC12
  Lane 4: Mouse embryonic stem cell
  Lane 5: NIH/3T3

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-NF-κB p65 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with NF-κB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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