Anti-LOXL2 antibody
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概述
- 产品描述Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E-cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation.
- 产品名称Anti-LOXL2 antibody
- 分子量87 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human LOXL2 aa 300-480.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Endoplasmic reticulum. Nucleus. Secreted.
- 其它名称LOR 2 antibody
LOR2 antibody
LOX L2 antibody
LOXL 2 antibody
LOXL2 antibody
LOXL2_HUMAN antibody
Lysyl oxidase homolog 2 antibody
Lysyl oxidase like 2 antibody
Lysyl oxidase like protein 2 antibody
Lysyl oxidase related 2 antibody
Lysyl oxidase related protein 2 antibody
Lysyl oxidase related protein WS9 14 antibody
Lysyl oxidase-like protein 2 antibody
Lysyl oxidase-related protein 2 antibody
Lysyl oxidase-related protein WS9-14 antibody
WS9 14 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:100
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of LOXL2 on A549 and A431 cell lysate using anti-LOXL2 antibody at 1/500 dilution.
Fig2: ICC staining LOXL2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining LOXL2 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig6: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-LOXL2 antibody. Counter stained with hematoxylin. The section was pretreated using heat mediated antigen retrieval with sodium citrate buffer (pH6) for 20 mins.
Fig8: Flow cytometric analysis of A549 cells with LOXL2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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