Anti-USP28 antibody
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概述
- 产品描述Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with isoform 1 of FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBW7) complex. In contrast, it does not interact with isoform 4 of FBXW7 (FBW7gamma) in the nucleolus, allowing MYC degradation and explaining the selective MYC degradation in the nucleolus. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
- 产品名称Anti-USP28 antibody
- 分子量122 kDa
- 种属反应性Human
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human USP28 aa 550-700.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus.
- 其它名称Deubiquitinating enzyme 28 antibody
KIAA1515 antibody
Ubiquitin carboxyl terminal hydrolase 28 antibody
Ubiquitin carboxyl-terminal hydrolase 28 antibody
ubiquitin carboxyl-terminal hydrolase 28 variant 1 antibody
Ubiquitin specific peptidase 28 antibody
Ubiquitin specific processing protease 28 antibody
Ubiquitin specific protease 28 antibody
Ubiquitin thioesterase 28 antibody
Ubiquitin thiolesterase 28 antibody
Ubiquitin-specific-processing protease 28 antibody
UBP28_HUMAN antibody
USP 28 antibody
USP28 antibody
USP28 protein antibody
more
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应用
WB: 1:200-1:1,000
FC: 1:50-1:100
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Fig1: Western blot analysis of USP28 on different cell lysates using anti-USP28 antibody at 1/500 dilution.
Positive control:
Lane 1: K562
Lane 2: A549
Lane 3: Daudi
Fig2: Flow cytometric analysis of MG-63 cells with USP28 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
