Anti-Rice Os01g0847700 protein antibody
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概述
- 产品描述Has oxidoreductase activity.
- 产品名称Anti-Rice Os01g0847700 protein antibody
- 分子量36 kDa
- 种属反应性Rice
- 验证应用WB,IF,IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide within Rice Os01g0847700 aa 292-316.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm.
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应用
WB:1:500-1:1000
IF:1:50-1:200
IHC-P:1:50-1:200
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Fig1: Western blot analysis of Rice Os01g0847700 protein on rice stem lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: IF staining of Rice Os01g0847700 protein in rice stem tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded rice stem tissue using anti-Rice Os01g0847700 protein antibody. Counter stained with hematoxylin.
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