Anti-ATG4A antibody
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概述
- 产品描述Cysteine protease ATG4A is an enzyme that in humans is encoded by the ATG4A gene. Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodelling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified.
- 产品名称Anti-ATG4A antibody
- 分子量45 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within Human ATG4A aa 87-314.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm.
- 其它名称AI627006 antibody
APG4 autophagy 4 homolog A antibody
Apg4a antibody
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ATG4 autophagy related 4 homolog A antibody
ATG4A antibody
ATG4A_HUMAN antibody
Atg4al antibody
AUT like 2 cysteine endopeptidase antibody
AUT-like 2 cysteine endopeptidase antibody
Autl2 antibody
Autophagin 2 antibody
Autophagin-2 antibody
Autophagy related 4A cysteine peptidase antibody
Autophagy related cysteine endopeptidase 2 antibody
Autophagy related protein 4 homolog A antibody
Autophagy-related cysteine endopeptidase 2 antibody
Autophagy-related protein 4 homolog A antibody
AV169859 antibody
Cysteine protease ATG4A antibody
hAPG4A antibody
MGC107179 antibody
more
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应用
WB:1:500-1:1000
IHC-P:1:50-1:200
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Fig1: Western blot analysis of ATG4A on rat bone marrow. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ATG4A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibodyfor 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
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