Anti-Connexin 45 / GJA7 / Cx45 antibody
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概述
- 产品描述Gap junction gamma-1 protein (GJC1), also known as gap junction alpha-7 protein (GJA7) and connexin 45 (Cx45) — is a protein that in humans is encoded by the GJC1 gene. Cx45 is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Alternatively spliced transcript variants encoding the same isoform have been described. GJs consist of 3 major connexin (Cx) isoforms—Cx43, Cx40, and Cx45—each of which are characterized by chamber-specific regional distribution and permeation properties. Cx45 is strongly expressed in the early embryonic myocardium and is required for cardiac development (1). Homozygous Cx45-deficient mice are embryonic lethal; however, in the adult heart, Cx45 is expressed mainly at the SA and AV nodes and is not essential for survival of adult mice. Disease-causing mutations in GJ gamma-1 protein (GJC1), which encodes Cx45, have not yet been identified.
- 产品名称Anti-Connexin 45 / GJA7 / Cx45 antibody
- 分子量Predicted band size: 45 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within Human Connexin 45 aa 280-410.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cell membrane, Gap junction.
- 其它名称Connexin 45 / GJA7 antibody
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应用
WB:1:500-1:1000
ICC:1:500-1:100
IHC-P:1:50-1:100
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Fig1: Western blot analysis of Connexin 45 / GJA7 / Cx45 on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: Anti-Connexin-45 Antibody;
Lane 2: Anti-Connexin-45 Antibody, pre-incubated with the
immunizaiton peptide.
Fig2: ICC staining Connexin 45 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: ICC staining Connexin 45 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Connexin 45 antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig4: Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Connexin 45 / GJA7 / Cx45 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues we
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