Anti-C22orf25 antibody

Anti-C22orf25 antibody

• 概述

• 产品名称Anti-C22orf25 antibody
• 分子量31 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Zebrafish
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human C22orf25 21-120/276
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 其它名称Uncharacterized protein C22orf25
  chromosome 22 open reading frame 25
  DKFZp 761 P 1121
  Hypothetical protein LOC128989
  TNG2_HUMAN.

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:Placenta (Mouse) Lysate at 40 ug
  Primary: Anti- C22orf25 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31kD
  Observed band size: 35kD

   

  

  Fig2: Sample:Lovo Cell (Human) Lysate at 30 ug
  Primary: Anti- C22orf25 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31kD
  Observed band size: 35kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (C22orf25) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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