Anti-SPOCK2 antibody

Anti-SPOCK2 antibody

• 概述

• 产品描述May participate in diverse steps of neurogenesis. Binds calcium.
• 产品名称Anti-SPOCK2 antibody
• 分子量45 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep
• 验证应用WB,IHC-P,ICC/IF
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human SPOCK2/Testican 2 61-160/424
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称Kazal-like domains proteoglycan 2
  CWCV
  FLJ97039
  KIAA0275
  SPARC/osteonectin
  Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2
  Sparc/osteonectin cwcv and kazal like domains proteoglycan 2
  SPOCK 2
  Spock2
  Testican 2
  Testican-2
  Testican2
  TICN 2
  TICN2
  TICN2_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IHC-F:1:400-800
  IF:1:100-500
  ICC/IF:1:100-500

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugatedat 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Sample: Hela Cell (Human) Lysate at 40 ug
  Primary: Anti-SPOCK2at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 45 kD
  Observed band size: 50 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！