Anti-SRF antibody

Anti-SRF antibody

• 概述

• 产品描述SRF is a transcription factor that binds to the serum response element (SRE), a short sequence of dyad symmetry located 300 bp to the 5' of the site of transcription initiation of some genes (such as FOS). Required for cardiac differentiation and maturation.
• 产品名称Anti-SRF antibody
• 分子量56 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human SRF 101-250/508
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nucleus.
• 其它名称c fos serum response element binding factor
  Serum Response Factor SRF
  c fos serum response element binding transcription factor
  ELK3
  ERP
  MCM 1
  MCM-1
  MCM1
  OTTHUMP00000039820
  SAP2
  Serum response factor
  SRF
  SRF serum response factor c fos serum response element binding transcription factor
  SRF_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SRF) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig2: Sample:
  A431(Human) Cell Lysate at 30 ug
  Hela(Human) Cell Lysate at 30 ug
  293T(Human) Cell Lysate at 30 ug
  Primary: Anti- SRFat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56 kD
  Observed band size: 52 kD

   

  

  Fig3: Sample: NIH/3T3 Cell Lysate at 30 ug
  Primary: Anti-SRF  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
  Predicted band size: 56 kD
  Observed band size: 54 kD

   

  

  Fig4: Sample: Muscle(Mouse) Lysate at 30 ug
  Primary: Anti- SRF at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
  Predicted band size: 56 kD
  Observed band size: 56 kD

   

  

  Fig5: Sample: Large intestine (Mouse) Lysate at 30 ug
  Primary: Anti- SRF at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/10000 dilution
  Predicted band size: 56 kD
  Observed band size: 54 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！