Anti-SUPT16H antibody
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概述
- 产品描述Component of the FACT complex, a general chromatinfactor that acts to reorganize nucleosomes. The FACT complex isinvolved in multiple processes that require DNA as a template suchas mRNA elongation, DNA replication and DNA repair. Duringtranscription elongation the FACT complex acts as a histonechaperone that both destabilizes and restores nucleosomalstructure. It facilitates the passage of RNA polymerase II andtranscription by promoting the dissociation of one histone H2A-H2Bdimer from the nucleosome, then subsequently promotes thereestablishment of the nucleosome following the passage of RNApolymerase II. The FACT complex is probably also involved inphosphorylation of 'Ser-392' of p53/TP53 via its association withCK2 (casein kinase II). Also involved in vitamin D-coupledtranscription regulation via its association with the WINACcomplex, a chromatin-remodeling complex recruited by vitamin Dreceptor (VDR), which is required for the ligand-bound VDR-mediatedtransrepression of the CYP27B1 gene (By similarity).
- 产品名称Anti-SUPT16H antibody
- 分子量120 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human SUPT16H/CDC68 601-700/1047
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus. Chromosome. Note=Colocalizes withRNA polymerase II on chromatin. Recruited to actively transcribedloci.
- 其它名称CDC68
Chromatin specific transcription elongation factor 140 kDa subunit
Chromatin-specific transcription elongation factor 140 kDa subunit
Facilitates chromatin transcription complex subunit SPT16
FACT 140 kDa subunit
FACT
FACT complex subunit SPT16
FACTP140
FLJ10857
FLJ14010
hSPT16
SP16H_HUMAN
Suppressor of Ty 16 homolog
Supt16h.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample: Placenta (Mouse) Lysate at 40 ug
Primary: Anti-SUPT16H at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 120 kD
Observed band size: 120 kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SUPT16H) Polyclonal Antibody, Unconjugated (at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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