Anti-TGF beta Receptor II antibody
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概述
- 产品描述Transmembrane serine/threonine kinase forming with the TGF-beta type I serine/threonine kinase receptor, TGFBR1, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFRB1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways.
- 产品名称Anti-TGF beta Receptor II antibody
- 分子量62 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Rabbit,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human TGF beta R2 241-330/567
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Single-pass type I membrane protein.
- 其它名称TGF beta R2
TGFBR2
TGF beta Receptor II
AAT 3
AAT3
FAA 3
FAA3
HNPCC6
MFS 2
MFS2
RIIC
TAAD 2
TAAD2
TbetaR II
TGF beta receptor type 2
TGF beta receptor type II
TGF beta type II receptor
TGFB R2
TGFbeta RII
TGFBR 2
TGFBR2
TGFR 2
TGFR2
Transforming growth factor beta receptor II
Transforming growth factor beta receptor type II
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-TGF beta Receptor II at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Fig2: Sample:
Brain(Mouse) Lysate at 30 ug
Brain(Rat) Lysate at 30 ug
Primary: Anti-TGF-beta-R2/TGFBR2 at 1/300 dilution
Secondary:IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Fig3: Sample: HepG2 Cell Lysate at 40 ug
Primary: Anti- TGF beta RECEPTOR Ⅱ(at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
