Anti-UCP-1 antibody

Anti-UCP-1 antibody

• 概述

• 产品描述Mitochondrial protein responsible for thermogenic respiration, a specialized capacity of brown adipose tissue and beige fat that participates to non-shivering adaptive thermogenesis to temperature and diet variations and more generally to the regulation of energy balance. Functions as a long-chain fatty acid/LCFA and proton symporter, simultaneously transporting one LCFA and one proton through the inner mitochondrial membrane. However, LCFAs remaining associated with the transporter via their hydrophobic tails, it results in an apparent transport of protons activated by LCFAs. Thereby, dissipates the mitochondrial proton gradient and converts the energy of substrate oxydation into heat instead of ATP. Regulates the production of reactive oxygen species/ROS by mitochondria.
• 产品名称Anti-UCP-1 antibody
• 分子量33 KDa
• 种属反应性Sheep,Human,Mouse,Rat,Dog,Cow,Horse,Rabbit
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human UCP-1 101-200/307
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Mitochondrion inner membrane; Multi-pass membrane protein.
• 其它名称Uncoupling Protein-1
  UCP1
  UCP 1
  Mitochondrial brown fat uncoupling protein 1
  mitochondrial brown fat uncoupling protein
  SLC25A7
  Thermogenin
  UCP 1
  UCP
  Uncoupling protein 1
  UCP1_HUMAN.
  more

• 应用

  WB:1:500-2000
  FC:1μg /test

•  

  

  Fig1: Blank control: Mouse spleen (blue).
  Primary Antibody:Rabbit Anti-UCP-1 antibody( Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (ER1918-37, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Thymus (Mouse) Lysate at 40 ug
  Primary: Anti- UCP-1at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 33 kD
  Observed band size: 33 kD

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