Anti-uPA Receptor antibody
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概述
- 产品描述The Urokinase receptor, also known as uPA receptor or uPAR or CD87 (Cluster of Differentiation 87), is a multidomain glycoprotein tethered to the cell membrane with a glycosylphosphotidylinositol (GPI) anchor. uPAR was originally identified as a saturable binding site for urokinase on the cell surface. uPAR is a part of the plasminogen activation system, which in the healthy body is involved in tissue reorganization events such as mammary gland involution and wound healing. An important mechanism in this degradation is the proteolysis cascade initiated by the plasminogen activation system. uPAR binds urokinase and thus restricts plasminogen activation to the immediate vicinity of the cell membrane. Thus uPAR seems to be an important player in the regulation of this process. However the components of the plasminogen activation system have been found to be highly expressed in many malignant tumors, indicating that tumors are able to hijack the system, and use it in metastasis. uPAR has been involved in various other non-proteolytical processes related to cancer, such as cell migration, cell cycle regulation, and cell adhesion. When uPA is bound to the receptor, there is cleavage between the GPI-anchor and the uPAR, releasing suPAR.
- 产品名称Anti-uPA Receptor antibody
- 分子量31 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human PLAUR 201-300/335
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A
- 亚细胞定位Isoform 1: Cell membrane; Lipid-anchor, GPI-anchor. Isoform 2: Secreted (Probable).
- 其它名称PLAUR
Plasminogen activator,urokinase receptor
Urokinase plasminogen activator surface receptor
Monocyte activation antigen Mo3
uPAR
U-PAR
MO3
UPAR
CD 87
CD87
CD87 antigen
MO 3
MO3
Monocyte activation antigen Mo3
Plasminogen activator urokinase receptor
U PAR
u plasminogen activator receptor
UPA receptor
UPAR
Urinary plasminogen activator receptor
URKR
Urokinase plasminogen activator receptor
Urokinase plasminogen activator surface receptor.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Tissue/cell: mouse adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-uPA Receptor Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample:
bone (Mouse) Lysate at 40 ug
Primary: Anti-uPA Receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 48 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
