Anti-RAR alpha antibody
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概述
- 产品描述Retinoids (RA) are metabolites of vitamin A (retinol) that are important signaling molecules during vertebrate development and tissue differentiation. RAs activate the retinoic acid receptor (RAR) and retinoid X receptor (RXR) nuclear transcription factor families. Most retinoid forms activate RAR family members, whereas RXR family members are activated by 9-cis-RA only. RAR family members, which include RARα, RARβ and RARγ, have a high affinity for all transretinoic acids and belong to the same class of nuclear transcription factors as thyroid hormone receptors, vitamin D3 receptor and ecdysone receptor. RAR isoforms are expressed in distinct patterns throughout development and in the mature organism. The human RARα gene maps to chromosome 17 and is implicated in the chromosomal translocation associated with acute promyelocytic leukemia (APL-M3). Specifically, the RARα gene is fused with the promyelocytic leukemia (PML) gene, which encodes the fusion protein PML/RARα. The PML/RARα fusion protein inhibits PML-dependent apoptotic pathways and halts myeloid differentiation at the promyelocytic stage.
- 产品名称Anti-RAR alpha antibody
- 分子量51 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus. Cytoplasm.
- 其它名称NR1B1 antibody
Nuclear mitotic apparatus protein retinoic acid receptor alpha fusion protein antibody
Nuclear receptor subfamily 1 group B member 1 antibody
Nucleophosmin retinoic acid receptor alpha fusion protein NPM RAR long form antibody
RAR alpha antibody
RAR antibody
RAR-alpha antibody
rara antibody
RARA_HUMAN antibody
RARalpha antibody
RARalpha1 antibody
Retinoic acid nuclear receptor alpha variant 1 antibody
Retinoic acid nuclear receptor alpha variant 2 antibody
Retinoic acid receptor alpha antibody
Retinoic acid receptor alpha polypeptide antibody
more
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应用
WB: 1:500
ICC: 1:500-1:2,000
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of RAR alpha on different lysates using anti-RAR alpha antibody at 1/500 dilution.
Positive control:
Lane 1: Hela
Lane 2: Human lung tissue
Fig2: ICC staining RAR alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining RAR alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining RAR alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brian tissue using anti-RAR alpha antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of MCF-7 cells with RAR alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the seco
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