Anti-ECM1 antibody
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概述
- 产品描述Involved in endochondral bone formation as negative regulator of bone mineralization. Stimulates the proliferation of endothelial cells and promotes angiogenesis. Inhibits MMP9 proteolytic activity.
- 产品名称Anti-ECM1 antibody
- 分子量59 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human ECM1 488-567/567
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Secreted, extracellular space, extracellular matrix.
- 其它名称Secretory Component Glycoprotein
ECM 1
Ecm1
ECM1_HUMAN
Extracellular matrix protein 1
Secretory component p85.
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-ECM1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 64 kD
Fig2: Sample:
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-ECM1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 64 kD
Fig3: Sample:
Skin(Mouse) Lysate at 40 ug
Primary: Anti-ECM1 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 64 kD
Fig4: Sample:
MDA-MB-231(Mouse) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-ECMat 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 65 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
