Anti-EP4 antibody
-
概述
- 产品描述Receptor for prostaglandin E2 (PGE2). The activity of this receptor is mediated by G(s) proteins that stimulate adenylate cyclase. Has a relaxing effect on smooth muscle. May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.
- 产品名称Anti-EP4 antibody
- 分子量53 KDa
- 种属反应性Human,Bee
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human EP4R/Prostaglandin E Receptor EP4 151-250/488
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane.
- 其它名称Prostaglandin E Receptor EP4
EP 4
EP4
EP-4
EP4R
MGC126583
PE2R4_HUMAN
PGE receptor EP4 subtype
PGE2 receptor EP4 subtype
Prostaglandin E receptor 4
Prostaglandin E receptor 4 subtype EP4
Prostaglandin E2 receptor
Prostaglandin E2 receptor EP4 subtype
Prostanoid EP4 receptor
PTGER 2
PTGER 4
PTGER2
PTGER4.
more
-
应用
WB:1:500-2000
FC:1μg/Test
-
Fig1: Sample:
Raji(Human) Cell Lysate at 30 ug
Primary: Anti-EP4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
Fig2: Blank control (blue line): Hela (fixed with 70% methanol (Overnight at -20℃) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody (green line): Rabbit Anti-EP4 antibody,Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC,Dilution: 1μg /test.
Fig3: Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-EP4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 53 kD
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!