Anti-CTLA4 antibody
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概述
- 产品描述Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
- 产品名称Anti-CTLA4 antibody
- 分子量21 KDa
- 种属反应性Human,Mouse,Rat,Dog,Cow,Rabbit,Sheep
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human CTLA-4/CD152 75-170/223
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation.
- 其它名称CD 152
CD152
CD152 antigen
Celiac disease 3
CELIAC3
CTLA 4
CTLA-4
Cytotoxic T cell associated 4
Cytotoxic T lymphocyte associated 4
Cytotoxic T lymphocyte associated antigen 4
Cytotoxic T lymphocyte associated protein 4
Cytotoxic T lymphocyte associated serine esterase 4
Cytotoxic T lymphocyte protein 4
Cytotoxic T-lymphocyte-associated antigen 4
Cytotoxic T-lymphocyte-associated protein 4
GSE
IDDM12.
more
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应用
WB:1:500-2000
FC:1μg/Test
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Fig1: Sample:
A549(Human) Cell Lysate at 30 ug
Primary: Anti-CTLA4at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 35 kD
Fig2: Sample:
Liver(Mouse) Cell Lysate at 30 ug
Primary: Anti-CTLA4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 35 kD
Fig3: Sample:
Liver(Rat) Cell Lysate at 30 ug
Primary: Anti-CTLA4at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 35 kD
Fig4: Sample: Lymph node (Mouse) Lysate at 40 ug
Primary: Anti-CTLA4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Fig5: Blank control: Raji(blue).
Primary Antibody: Rabbit Anti-CTLA4 antibod, Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig6: Sample: K562 Cell (Human) Lysate at 40 ug
Primary: Anti-CTLA4 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 21 kD
Fig7: Blank control: Hela(blue), the cells were fixed with 2% paraformaldehyde (10 min)
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Prim
Fig8: Blank control(blue):Mouse Spleen Cells(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody: Rabbit Anti- Phospho-c-Fos (Thr325)/FITC Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Contr
Fig9: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min) ).
Primary Antibody: Rabbit Anti-CTLA4/FITC Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
