Anti-Dopamine D3 receptor antibody

Anti-Dopamine D3 receptor antibody

• 概述

• 产品名称Anti-Dopamine D3 receptor antibody
• 分子量44 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC/IF
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from mouse DRD3 352-446/446
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Multi-pass membrane protein. Note=Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 min
• 数据链接SwissProt: P30728 Mouse
  
• 其它名称DRD3
  3dopamine receptor
  D
  Dopamine receptor D3
  DRD3
  Dopamine receptor 3
  ETM1
  FET1
  D(3) dopamine receptor
  DRD3
  DRD3_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:200-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Dopamine D3 receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Brain (Mouse) Lysate at 40 ug
  Brain (Rat) Lysate at 40 ug
  Primary: Anti-Dopamine D3 receptorat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 44 kD
  Observed band size: 48 kD

   

  

  Fig3: Sample:
  HepG2 Cell Lysate at 30 ug
  U251 Cell Lysate at 30 ug
  Primary: Anti-Dopamine D3 receptor at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 44 kD
  Observed band size: 48 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！