Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody
-
概述
- 产品描述The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases(MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
- 产品名称Anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody
- 分子量42 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated Synthesised phosphopeptide derived from human p38 MAPK around the phosphorylation site of Thr180/Tyr182: M(p-T)G(p-Y)VA.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃. Avoid repeated freeze/thaw cycles.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式ProA affinity purified.
- 亚细胞定位Cytoplasm. Nucleus.
-
应用
WB: 1:500-1:2,000
IHC-P: 1:400-1:800
FC: 1:50
-
Fig1: Western blot analysis of Phospho-P38 MAPK (Thr180 + Tyr182) on different tissue lysates using anti-Phospho-P38 MAPK (Thr180 + Tyr182) at 1/300 dilution.
Positive control:
Line 1: Mouse muscle tissue
Line 2: Rat muscle tissue
Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Phospho-P38 MAPK (Thr180 + Tyr182) antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of HepG2 cells with Phospho-P38 MAPK (Thr180 + Tyr182) antibody at 1/50 dilution (green) compared with blank control (blue) and isotype control (orange). Goat anti-rabbit IgG-FITC was used as the secondary antibody (white blue).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
