Anti-ADAMTS5 antibody
-
概述
- 产品描述Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan (PubMed:16133547, PubMed:18992360). Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in development of brown adipose tissue and browning of white adipose tissue. Plays an important role for T-lymphocyte migration from draining lymph nodes following viral infection.
- 产品名称Anti-ADAMTS5 antibody
- 分子量102 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Aggrecan 2 511-610/930
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A
- 亚细胞定位Secreted, extracellular space, extracellular matrix.
- 其它名称ADAMTS5
A disintegrin and metalloproteinase with thrombospondin motifs 5
A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5
A Disintigrin And Metalloproteinase with ThromboSpondin motif-5
ADAM metallopeptidase with thrombospondin type 1 motif 5
ADAM TS 11
ADAM TS 5
ADAM TS5
ADAMTS 11
ADAMTS 5
ADAMTS11
ADMP 2
ADMP2
Aggrecanase 2
aggrecanase-2
FLJ36738
Implantin
ThromboSpondin motif-5.
more
-
应用
WB:1:500-2000
IHC-P:1:400-800
-
Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Aggrecanase-2/ADAMTS5 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Sample:Liver (Mouse) Lysate at 40 ug
Primary: Anti-ADAMTS5at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 102 kD
Observed band size: 102 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
