Anti-Cleaved-Notch 2 (D1733) antibody

Anti-Cleaved-Notch 2 (D1733) antibody

• 概述

• 产品描述This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cle
• 产品名称Anti-Cleaved-Notch 2 (D1733) antibody
• 分子量100/130 kDa
• 种属反应性Human, Mouse, Rat
• 验证应用IF, WB, IHC-P
• 抗体类型兔多抗
• 免疫原The antiserum was produced against synthesized peptide derived from human Notch 2. AA range:1684-1733
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• 亚型IgG
• 纯化方式Immunogen affinity purified.
• 亚细胞定位Cell membrane, Nucleus, Cytoplasm.
• 其它名称NOTCH2 antibody; Neurogenic locus notch homolog protein 2 antibody; Notch 2 antibody; hN2 antibody;

• 应用

  IF: 1:50-200 WB: 1:500-1:2000
  IHC-P: 1:100-1:300

•  

  

  Fig1: Immunofluorescence analysis of rat-lung tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1,Cleaved-Notch 2 (D1733) Polyclonal Antibody was diluted at 1:200(4°C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.

   

  

  Fig3: Western Blot analysis of various cells using Cleaved-Notch 2 (D1733) Polyclonal Antibody diluted at 1：500

   

  

  Fig4: The picture was kindly provided by our customer,antibody was diluted at 1:500

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