Anti-PTP1B antibody
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概述
- 产品描述The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
- 产品名称Anti-PTP1B antibody
- 分子量50 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified
- 亚细胞定位Endoplasmic reticulum.
- 其它名称PTP1B antibody
Non receptor tyrosine phosphatase 1 antibody
Protein phosphotyrosylphosphatase 1B antibody
Protein tyrosine phosphatase 1B antibody
Protein tyrosine phosphatase non receptor type 1 antibody
Protein tyrosine phosphatase placental antibody
Protein-tyrosine phosphatase 1B antibody
PTN1_HUMAN antibody
PTP 1B antibody
PTP-1B antibody
PTPN 1 antibody
PTPN1 antibody
Tyrosine protein phosphatase non receptor type 1 antibody
Tyrosine-protein phosphatase non-receptor type 1 antibody
more
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应用
WB: 1:1,000-1;5,000
ICC: 1:500-1:2,000
IHC-P: 1:100-1:500
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Fig1: Western blot analysis of PTP1B on recombinant protein lysate using anti-PTP1B antibody at 1/5,000 dilution.
Fig2: ICC staining PTP1B in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PTP1B in Siha cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse fallopian tubes tissue using anti-PTP1B antibody. Counter stained with hematoxylin.
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