Anti-HIF1 alpha antibody
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概述
- 产品描述Cell growth and viability is compromised by oxygen deprivation (hypoxia). Hypoxia-inducible factors, including HIF-1α, HIF-1β (also designated Arnt 1), EPAS-1 (also designated HIF-2α) and HIF-3α, induce glycolysis, erythropoiesis and angiogenesis in order to restore oxygen homeostasis. Hypoxia-inducible factors are members of the Per-Arnt-Sim (PAS) domain transcription factor family. In response to hypoxia, HIF-1α is upregulated and forms a heterodimer with Arnt 1 to form the HIF-1 complex. The HIF-1 complex recognizes and binds to the hypoxia responsive element (HRE) of hypoxia-inducible genes, thereby activating transcription. Hypoxia-inducible expression of some genes such as Glut-1, p53, p21 or Bcl-2, is HIF-1α dependent, whereas expression of others, such as p27, GADD 153 or HO-1, is HIF-1α independent. EPAS-1 and HIF-3α have also been shown to form heterodimeric complexes with Arnt 1 in response to hypoxia.
- 产品名称Anti-HIF1 alpha antibody
- 分子量93 kDa
- 种属反应性Human, Mouse
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称ARNT interacting protein antibody
ARNT-interacting protein antibody
Basic helix loop helix PAS protein MOP1 antibody
Basic-helix-loop-helix-PAS protein MOP1 antibody
bHLHe78 antibody
Class E basic helix-loop-helix protein 78 antibody
HIF 1A antibody
HIF 1alpha antibody
HIF-1-alpha antibody
HIF1 A antibody
HIF1 Alpha antibody
HIF1 antibody
HIF1-alpha antibody
HIF1A antibody
HIF1A_HUMAN antibody
Hypoxia inducible factor 1 alpha antibody
Hypoxia inducible factor 1 alpha isoform I.3 antibody
Hypoxia inducible factor 1 alpha subunit antibody
Hypoxia inducible factor 1 alpha subunit basic helix loop helix transcription factor antibody
Hypoxia inducible factor 1, alpha subunit (basic helix loop helix transcription factor) antibody
Hypoxia inducible factor1alpha antibody
Hypoxia-inducible factor 1-alpha antibody
Member of PAS protein 1 antibody
Member of PAS superfamily 1 antibody
Member of the PAS Superfamily 1 antibody
MOP 1 antibody
MOP1 antibody
PAS domain-containing protein 8 antibody
PASD 8 antibody
PASD8 antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of HIF-1 alpha on mouse small intestine tissue lysate using anti-HIF-1 alpha antibody at 1/500 dilution.
Fig2: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining HIF-1 alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining HIF-1 alpha in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
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