Anti-APE1 antibody
-
概述
- 产品描述The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.
- 产品名称Anti-APE1 antibody
- 分子量36 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein with Human APE1 aa 1-250.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Nucleus. Endoplasmic reticulum.
- 其它名称AP endonuclease 1 antibody
AP endonuclease class I antibody
AP lyase antibody
APE 1 antibody
APE antibody
APE-1 antibody
APEN antibody
APEX 1 antibody
APEX antibody
APEX nuclease (multifunctional DNA repair enzyme) 1 antibody
Apex nuclease 1 antibody
APEX nuclease antibody
APEX1 antibody
APEX1_HUMAN antibody
Apurinic endonuclease antibody
Apurinic-apyrimidinic endonuclease 1 antibody
Apurinic/apyrimidinic (abasic) endonuclease antibody
Apurinic/apyrimidinic endonuclease 1 antibody
Apurinic/apyrimidinic exonuclease antibody
APX antibody
BAP1 antibody
Deoxyribonuclease (apurinic or apyrimidinic) antibody
DNA (apurinic or apyrimidinic site) lyase antibody
DNA-(apurinic or apyrimidinic site) lyase, mitochondrial antibody
EC 4.2.99.18 antibody
HAP 1 antibody
HAP1 antibody
Human Apurinic endonuclease 1 antibody
MGC139790 antibody
Multifunctional DNA repair enzyme antibody
Redox factor 1 antibody
Redox factor-1 antibody
REF 1 antibody
REF 1 protein antibody
REF-1 antibody
REF1 antibody
REF1 protein antibody
more
-
应用
WB: 1:500-1:2,000
ICC: 1:500-1:1,000
IHC-P: 1:100-1:500
FC: 1:50-1:100
-
Fig1: Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution.
Positive control:
Lane 1: HL-60
Lane 2: Human skin tissue
Lane 3: MCF-7
Lane 4: Mouse placenta tissue
Fig2: ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining APE1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining APE1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-APE1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-APE1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-APE1 antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of A549 cells with APE1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
