Anti-Dnmt3b antibody
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概述
- 产品描述Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.
- 产品名称Anti-Dnmt3b antibody
- 分子量96 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human Dnmt3b aa 1-50.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus.
- 其它名称Cytosine 5methyltransferase 3B antibody
DNA antibody
DNA (cytosine 5) methyltransferase 3 beta antibody
DNA (cytosine 5)-methyltransferase 3B antibody
DNA (cytosine-5)-methyltransferase 3B antibody
DNA methyltransferase HsaIIIB antibody
DNA MTase HsaIIIB antibody
DNM3B_HUMAN antibody
Dnmt3b antibody
EC 2.1.1.37 antibody
ICF antibody
ICF1 antibody
M.HsaIIIB antibody
MGC124407 antibody
RP23-89H14.3 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Dnmt3b on K562 cell lysate using anti-Dnmt3b antibody at 1/500 dilution.
Fig2: ICC staining Dnmt3b in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Dnmt3b antibody. Counter stained with hematoxylin.
Fig5: Flow cytometric analysis of K562 cells with Dnmt3b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
