Anti-T-bet antibody
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概述
- 产品描述T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T box expressed in T cells) is a Th1-specific T box transcription factor that controls the expres-sion of the Th1 cytokine, IFN-γ. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. T-bet is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.
- 产品名称Anti-T-bet antibody
- 分子量58 kDa
- 种属反应性Human,Mouse
- 验证应用WB,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within human T-bet aa 480-530.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Nucleus.
- 其它名称T bet antibody
T box 21 antibody
T box expressed in T cells antibody
T box protein 21 antibody
T box transcription factor TBX21 antibody
T cell specific T box transcription factor antibody
T cell specific T box transcription factor T bet antibody
T PET antibody
T-box protein 21 antibody
T-box transcription factor TBX21 antibody
T-cell-specific T-box transcription factor T-bet antibody
TBET antibody
TBLYM antibody
TBX 21 antibody
Tbx21 antibody
TBX21_HUMAN antibody TPET antibody
Transcription factor TBLYM antibody
more
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应用
WB: 1:500
FC: 1:50-1:100
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Fig1: Western blot analysis of T-bet on mouse marrow tissue lysate using anti-T-bet antibody at 1/500 dilution.
Fig2: Flow cytometric analysis of Jurkat cells with T-bet antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
