Anti-SUFU antibody
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概述
- 产品描述Su(fu) (for Suppressor-of-Fused) is a key negative regulator in the vertebrate Hedgehog signaling pathway. Su(fu) interacts with genes encoding proteins in this signal transduction pathway. In Drosophila, Intracellular transduction of the Hedgehog pathway involves the release of a large complex containing Su(fu). Su(fu) inhibits the activity of the transcription factor Gli1 and interacts with Gli2, Gli3 and the serine/threonine kinase Fused. Su(fu) is widely expressed in adult and embryonic tissues with higher expression in tissues patterned by hegdehog signaling. The Su(fu) gene locus maps to a region that is deleted in glioblastomas, prostate cancer, malignant melanoma and endometrial cancer.
- 产品名称Anti-SUFU antibody
- 分子量54 kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein with human SUFU aa 1-250.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称OTTHUMP00000020374 antibody
OTTHUMP00000020377 antibody
OTTHUMP00000020379 antibody
PRO1280 antibody
SU FU antibody
SU(F)U antibody
Su(fu) antibody
SUFU antibody
SUFU negative regulator of hedgehog signaling antibody
SUFU_HUMAN antibody
SUFUH antibody
SUFUXL antibody
Suppressor of fused homolog (Drosophila) antibody
Suppressor of fused homolog antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of SUFU on 293 cell lysate using anti-SUFU antibody at 1/500 dilution.
Fig2: ICC staining SUFU in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining SUFU in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SUFU antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SUFU antibody. Counter stained with hematoxylin.
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