Anti-Kir3.4 antibody
-
概述
- 产品描述This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium.
- 产品名称Anti-Kir3.4 antibody
- 分子量48 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within N-terminal human Kir3.4.
- 偶联Non-conjugated
-
性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Membrane.
- 其它名称Cardiac ATP sensitive potassium channel antibody
Cardiac inward rectifier antibody
CIR antibody
G protein activated inward rectifier potassium channel 4 antibody
G protein-activated inward rectifier potassium channel 4 antibody
GIRK 4 antibody
GIRK-4 antibody
GIRK4 antibody
Heart KATP channel antibody
Inward rectifier K(+) channel Kir3.4 antibody
Inward rectifier K+ channel KIR3.4 antibody
Inward rectifier potassium channel KIR3.4 antibody
inwardly rectifying subfamily J member 5 antibody
IRK-4 antibody
IRK5_HUMAN antibody
KATP 1 antibody
KATP-1 antibody
KATP1 antibody
KCNJ 5 antibody
Kcnj5 antibody
KIR 3.4 antibody
KIR3.4 antibody
LQT13 antibody
Potassium channel antibody
Potassium channel inwardly rectifying subfamily J member 5 antibody
Potassium inwardly rectifying channel J5 antibody
Potassium inwardly rectifying channel subfamily J member 5 antibody
potassium voltage-gated channel subfamily J member 5 antibody
more
-
应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
-
Fig1: Western blot analysis of Kir3.4 on different lysates using anti-Kir3.4 antibody at 1/500 dilution.
Positive control:
Lane 1: Mouse heart
Lane 2: Rat heart
Fig2: ICC staining Kir3.4 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Kir3.4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Kir3.4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir3.4 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of HepG2 cells with Kir3.4 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the sec
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
