Anti-CD162 antibody

Anti-CD162 antibody

• 概述

• 产品描述A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

• 产品名称Anti-CD162 antibody

• 分子量41 KDa

• 种属反应性Human

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PSGL-1 251-350/412

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein.

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• ​其它名称CD 162
  CD162
  CD162 antigen
  CLA
  Cutaneous lymphocyte associated associated antigen
  P selectin glycoprotein ligand 1
  P selectin glycoprotein ligand 1 precursor
  P-selectin glycoprotein ligand 1
  PSGL 1
  PSGL-1
  PSGL1
  Selectin P ligand
  SELPL_HUMAN
  SELPLG.

  more

• 应用

  WB:1:500-2000
  FC:1μg /test

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  Fig1: Sample:
  Jurkat(Human) Cell Lysate at 30 ug
  MOLT-4(Human) Cell Lysate at 30 ug
  Primary: Anti-CD162 at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 41 kD
  Observed band size: 41 kD

   

  

  Fig2: Sample:
  Raji(Human) Cell Lysate at 30 ug
  U937(Human) Cell Lysate at 30 ug
  Primary: Anti-CD162  at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 41 kD
  Observed band size: 41 kD

   

  

  Fig3: Blank control: U937(blue).
  Primary Antibody: Rabbit Anti-CD162 antibody(, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG (orange) ,used under the same conditions.
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min).Primary antibody (, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 10% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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