Anti-CD200R antibody

Anti-CD200R  antibody

• 概述

• 产品描述Receptor for the CD200/OX2 cell surface glycoprotein. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200.

• 产品名称Anti-CD200R antibody

• 分子量34 KDa

• 种属反应性Human

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD200R 151-250/325

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Secreted and Cell membrane.

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• ​其它名称CD 200R
  CD200 cell surface glycoprotein receptor
  CD200 receptor 1
  Cd200r1
  Cell surface glycoprotein CD200 receptor 1
  Cell surface glycoprotein OX2 receptor 1
  Cell surface glycoprotein OX2 receptor
  cell surface glycoprotein receptor CD200
  CRTR 2
  CRTR2
  HCRTR2
  MO2R1_HUMAN
  MOX2 receptor
  MOX2R
  OX2R.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:2μg/Test

•  

  

  Fig1: Sample:hela(Human) Cell Lysate at 30 ug
  Raji(Human) Cell Lysate at 30 ug
  Primary: Anti-CD200R at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 33 kD
  Observed band size: 58 kD

   

  

  Fig2: Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD200R at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 33 kD Observed band size: 53 kD

   

  

  Fig3: Blank control:Hepg2 Cells(blue).
  Primary Antibody: Rabbit Anti-CD200R/AF647 Conjugated antibody, Dilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

   

  

  Fig4: Sample:
  HepG2(Human) Cell Lysate at 30 ug
  Primary: Anti-CD200R at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 34 kD
  Observed band size: 34 kD

   

  

  Fig5: Sample:
  U87MG(Human) Cell Lysate at 30 ug
  Primary: Anti-CD200R  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 34 kD
  Observed band size: 34 kD

   

  

  Fig6: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CD200R) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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