Anti-CD62p antibody

Anti-CD62p antibody

• 概述

• 产品描述Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

• 产品名称Anti-CD62p antibody

• 分子量88 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from mouse P-selectin 701-768/768

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein.

•  

• ​其它名称CD62 antigen-like family member P
  GMP 140
  GMP-140
  GMRP
  Granule membrane protein 140
  Granulocyte membrane protein
  GRMP
  LECAM 3
  LECAM3
  Leukocyte endothelial cell adhesion molecule 3
  Leukocyte-endothelial cell adhesion molecule 3
  LYAM3_HUMAN
  P Selectin
  P-selectin
  PADGEM
  Platelet activation dependent granule-external membrane protein
  PSEL
  sp-selectin.

  more

• 应用

  IHC-P:1:400-800
  FC:1μg/Test

•  

  

  Fig1: Blank control: HUVEC cells(blue).
  Primary Antibody:Rabbit Anti-CD62p antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) .Primary antibody (, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig2: Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-P-selectin Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

•  

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！