Anti-CEP192 antibody

Anti-CEP192 antibody

• 概述

• 产品描述Centrosomal protein of 192 kDa, also known as Cep192, is a protein that in humans is encoded by the CEP192 gene. It is the homolog of the C. elegans and D. melanogaster gene SPD-2. Cep192 is a major regulator of pericentriolar material recruitment, centrosome maturation, and centriole duplication in mammalian cells. It stimulates the formation of the scaffolding upon which gamma tubulin ring complexes and other proteins involved in microtubule nucleation and spindle assembly become functional during mitosis.

• 产品名称Anti-CEP192 antibody

• 分子量213 KDa

• 种属反应性Human,Mouse

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CEP192 51-150/1941

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Required for mitotic centrosome and spindle assembly. Appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

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• 其它名称Centrosomal protein 192kDa
  Centrosomal protein of 192 kDa
  Cep192
  KIAA1569
  PP8407
  PPP1R62
  CE192_HUMAN
  Protein phosphatase 1, regulatory subunit 62.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Tissue/cell: human liver cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-CEP192 Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Sample:
  Spleen (Mouse) Lysate at 40 ug
  Primary: Anti-CEP192  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 213 kD
  Observed band size: 150 kD

   

  

  Fig3: Sample:
  Testis (Mouse) Lysate at 40 ug
  Primary: Anti-CEP192at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 213 kD
  Observed band size: 150 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！