Anti-Collagen III antibody

Anti-Collagen III antibody

• 概述

• 产品描述Type III Collagen is a homotrimer, or a protein composed of three identical peptide chains (monomers), each called an alpha 1 chain of type III collagen. Formally, the monomers are called collagen type III, alpha-1 chain and in humans are encoded by the COL3A1 gene. Type III collagen is one of the fibrillar collagens whose proteins have a long, inflexible, triple-helical domain. In addition to being an integral structural component of many organs, type III collagen is also an important regulator of the diameter of type I and II collagen fibrils. Type III collagen is also known to facilitate platelet aggregation through its binding to platelets and therefore, play an important role in blood clotting. Type III collagen is found as a major structural component in hollow organs such as large blood vessels, uterus and bowel. It is also found in many other tissues together with type I collagen.

• 产品名称Anti-Collagen III antibody

• 分子量117 KDa

• 种属反应性Human,Mouse,Rat,Cow

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain 801-900/1466

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Secreted, extracellular space, extracellular matrix.

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• ​其它名称COL 3A1
  COL3A1
  Collagen alpha 1(III) chain
  Collagen III alpha 1 chain precursor
  Collagen III alpha 1 polypeptide
  Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant)
  Collagen type III alpha 1
  Collagen type III alpha
  EDS4A
  Ehlers Danlos syndrome type IV, autosomal dominant
  Fetal collagen
  Type III collagen
  CO3A1_HUMAN
  Collagen alpha-1(III) chain
  Type III collagen
  type III preprocollagen alpha 1 chain..

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500

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  Fig1: Sample:
  Hela Cell (Human) Lysate at 40 ug
  Primary: Anti- Collagen III at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 117 kD
  Observed band size: 130 kD

   

  

  Fig2: Tissue/cell: human colon cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！