Anti-HIF Prolyl Hydroxylases antibody

Anti-HIF Prolyl Hydroxylases antibody

• 概述

• 产品描述Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF1A at 'Pro-402' and 'Pro-564'. May function as a cellular oxygen sensor and, under normoxic conditions, may target HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.

• 产品名称Anti-HIF Prolyl Hydroxylases antibody

• 分子量55 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human PH-4 201-300/502

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Endoplasmic reticulum membrane; Single-pass type II membrane protein.

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• ​其它名称PHD4/prolyl hydroxylase
  EGLN4
  FLJ20262
  HIF prolyl hydroxylase PH4
  HIF-PH4
  HIF-prolyl hydroxylase 4
  HIFPH4
  HPH-4
  Hypoxia inducible factor prolyl 4 hydroxylase
  Hypoxia inducible factor prolyl hydroxylase
  Hypoxia-inducible factor prolyl hydroxylase 4
  P4H TM
  P4H with transmembrane domain
  P4H-TM
  P4htm
  P4HTM_HUMAN
  PH 4
  PH4
  PHD4
  Proline 4 hydroxylase
  Prolyl 4 hydroxylase transmembrane (endoplasmic reticulum)
  Prolyl hydroxlase domain containing 4
  Transmembrane prolyl 4-hydroxylase.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HIF Prolyl Hydroxylases) Polyclonal Antibody, Unconjugated  at 1:200 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Muscle (Mouse) Lysate at 40 ug
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti- HIF Prolyl Hydroxylases at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

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